PrimePCR™ Probe Assay: A1BG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051164
Assay Design:   exonic
Chromosome Location:   19:58863686-58863815question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000291270 ENST00000600757 ENST00000343373 ENST00000447742 ENST00000458663 ENST00000354227 ENST00000375180 ENST00000328235 ENST00000477084 ENST00000369733 ENST00000263100 ENST00000600966 ENST00000594687 ENST00000600333 ENST00000601479

Gene Information

The protein encoded by this gene is a plasma glycoprotein of unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins. [provided by RefSeq Jul 2008]

Gene Symbol:   A1BG
Gene Name:   alpha-1-B glycoprotein
Aliases:   A1B, ABG, DKFZp686F0970, GAB, HYST2477
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000121410
Entrez:   1
UniGene:   Hs.529161
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.240000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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