PrimePCR™ Probe Assay: INSL4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051107
Assay Design:   exonic
Chromosome Location:   9:5233729-5233849question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000239316

Gene Information

INSL4 encodes the insulin-like 4 protein a member of the insulin superfamily. INSL4 encodes a precursor that undergoes post-translational cleavage to produce 3 polypeptide chains A-C that form tertiary structures composed of either all three chains or just the A and B chains. Expression of INSL4 products occurs within the early placental cytotrophoblast and syncytiotrophoblast. [provided by RefSeq Jul 2008]

Gene Symbol:   INSL4
Gene Name:   insulin-like 4 (placenta)
Aliases:   EPIL, PLACENTIN
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000120211
Entrez:   3641
UniGene:   Hs.418506
Chromosome Mapping:   9p24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.350000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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