PrimePCR™ Probe Assay: CRHR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051102
Assay Design:   exonic
Chromosome Location:   17:43910834-43911103question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000550606 ENST00000548791 ENST00000552724 ENST00000551817 ENST00000314537 ENST00000352855 ENST00000577353 ENST00000398285 ENST00000580876 ENST00000339069 ENST00000293493

Gene Information

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress reproduction immune response and obesity. Alternative splicing results in multiple transcript variants one of which represents a read-through transcript with the neighboring gene MGC57346. [provided by RefSeq Jan 2012]

Gene Symbol:   CRHR1
Gene Name:   corticotropin releasing hormone receptor 1
Aliases:   CRF-R, CRF-R-1, CRF-R1, CRF1, CRFR-1, CRFR1, CRH-R-1, CRH-R1, CRH-R1h, CRHR, CRHR1L, CRHR1f
RefSeq:   NC_000017.10 NG_009902.1 NT_010783.15 NT_167251.1
Ensembl:   ENSG00000120088
Entrez:   1394
UniGene:   Hs.417628
Chromosome Mapping:   17q12-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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