PrimePCR™ Probe Assay: SMNDC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051095
Assay Design:   exonic
Chromosome Location:   10:112053929-112054039question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000369603 ENST00000369592

Gene Information

This gene is a paralog of SMN1 gene which encodes the survival motor neuron protein mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. The protein encoded by this gene is a nuclear protein that has been identified as a constituent of the spliceosome complex. This gene is differentially expressed with abundant levels in skeletal muscle and may share similar cellular function as the SMN1 gene. [provided by RefSeq Jul 2008]

Gene Symbol:   SMNDC1
Gene Name:   survival motor neuron domain containing 1
Aliases:   SMNR, SPF30
RefSeq:   NC_000010.10 NG_008284.1 NT_030059.13
Ensembl:   ENSG00000119953
Entrez:   10285
UniGene:   Hs.632093
Chromosome Mapping:   10q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.520000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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