PrimePCR™ Probe Assay: DCAF4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051076
Assay Design:   exonic
Chromosome Location:   14:73412612-73412707question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000358377 ENST00000353777 ENST00000394234 ENST00000509153 ENST00000555042 ENST00000553457

Gene Information

This gene encodes a WD repeat-containing protein that interacts with the Cul4-Ddb1 E3 ligase macromolecular complex. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Jul 2009]

Gene Symbol:   DCAF4
Gene Name:   DDB1 and CUL4 associated factor 4
Aliases:   DKFZp434K114, MGC20547, MGC46524, WDR21, WDR21A
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000119599
Entrez:   26094
UniGene:   Hs.331491
Chromosome Mapping:   14q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.390000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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