PrimePCR™ Probe Assay: RAB3GAP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RAB3GAP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RAB3GAP2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051056
Assay Design:   exonic
Chromosome Location:   1:220379268-220383757question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000358951 ENST00000484658

Gene Information

The protein encoded by this gene belongs to the RAB3 protein family members of which are involved in regulated exocytosis of neurotransmitters and hormones. This protein forms the Rab3 GTPase-activating complex with RAB3GAP1 where it constitutes the regulatory subunit whereas the latter functions as the catalytic subunit. This gene has the highest level of expression in the brain consistent with it having a key role in neurodevelopment. Mutations in this gene are associated with Martsolf syndrome.[provided by RefSeq Oct 2009]

Gene Symbol:   RAB3GAP2
Gene Name:   RAB3 GTPase activating protein subunit 2 (non-catalytic)
Aliases:   DKFZp434D245, FLJ14579, KIAA0839, RAB3-GAP150, RAB3GAP150, p150
RefSeq:   NC_000001.10 NG_015837.1 NT_167186.1
Ensembl:   ENSG00000118873
Entrez:   25782
Chromosome Mapping:   1q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996100
y-intercept 35.680000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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