PrimePCR™ Probe Assay: CNR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CNR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CNR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051039
Assay Design:   exonic
Chromosome Location:   6:88853756-88853889question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000369501 ENST00000369499 ENST00000549890 ENST00000362094 ENST00000468898 ENST00000428600 ENST00000535130 ENST00000537554 ENST00000549716

Gene Information

This gene encodes one of two cannabinoid receptors. The cannabinoids principally delta-9-tetrahydrocannabinol and synthetic analogs are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family which inhibit adenylate cyclase activity in a dose-dependent stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq May 2009]

Gene Symbol:   CNR1
Gene Name:   cannabinoid receptor 1 (brain)
Aliases:   CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000118432
Entrez:   1268
Chromosome Mapping:   6q14-q15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.080000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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