PrimePCR™ Probe Assay: NEK2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051012
Assay Design:   exonic
Chromosome Location:   1:211842663-211843717question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000366999 ENST00000366998 ENST00000540251

Gene Information

This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome and undetectable during G1 phase but accumulates progressively throughout the S phase reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq Feb 2011]

Gene Symbol:   NEK2
Gene Name:   NIMA (never in mitosis gene a)-related kinase 2
Aliases:   HsPK21, NEK2A, NLK1
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000117650
Entrez:   4751
UniGene:   Hs.153704
Chromosome Mapping:   1q32.2-q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.950000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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