PrimePCR™ Probe Assay: PRRC2C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051001
Assay Design:   exonic
Chromosome Location:   1:171557634-171560848question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000367742 ENST00000338920 ENST00000495585 ENST00000392078 ENST00000426496

Gene Information

Description Not Available

Gene Symbol:   PRRC2C
Gene Name:   proline-rich coiled-coil 2C
Aliases:   BAT2-iso, BAT2D1, BAT2L2, XTP2
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000117523
Entrez:   23215
UniGene:   Hs.494614
Chromosome Mapping:   1q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.240000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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