PrimePCR™ Probe Assay: GBP3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050989
Assay Design:   exonic
Chromosome Location:   1:89473531-89474777question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000370481 ENST00000461384 ENST00000445969 ENST00000370466

Gene Information

This gene encodes a member of the guanylate-binding protein (GBP) family. GBPs specifically bind guanine nucleotides (GMP GDP and GTP) and contain two of the three consensus motifs found in typical GTP-binding proteins. The encoded protein interacts with a member of the germinal center kinase family. [provided by RefSeq Jul 2008]

Gene Symbol:   GBP3
Gene Name:   guanylate binding protein 3
Aliases:   DKFZp686E0974, DKFZp686L15228, FLJ10961
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000117226
Entrez:   2635
UniGene:   Hs.720167
Chromosome Mapping:   1p22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.130000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download