PrimePCR™ Probe Assay: RRAGC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050976
Assay Design:   exonic
Chromosome Location:   1:39305207-39305354question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000373001

Gene Information

RRAGC is a monomeric guanine nucleotide-binding protein or G protein. By binding GTP or GDP small G proteins act as molecular switches in numerous cell processes and signaling pathways.[supplied by OMIM Apr 2004]

Gene Symbol:   RRAGC
Gene Name:   Ras-related GTP binding C
Aliases:   FLJ13311, GTR2, RAGC
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000116954
Entrez:   64121
UniGene:   Hs.532461
Chromosome Mapping:   1p34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.160000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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