PrimePCR™ Probe Assay: CFHR3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050965
Assay Design:   exonic
Chromosome Location:   1:196748975-196757346question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000367429 ENST00000359637 ENST00000439155 ENST00000367425 ENST00000471440 ENST00000391985

Gene Information

The protein encoded by this gene is a secreted protein which belongs to the complement factor H-related protein family. It binds to heparin and may be involved in complement regulation. Mutations in this gene are associated with decreased risk of age-related macular degeneration and with an increased risk of atypical hemolytic-uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   CFHR3
Gene Name:   complement factor H-related 3
Aliases:   CFHL3, DOWN16, FHR-3, FHR3, HLF4
RefSeq:   NC_000001.10 NG_005811.1 NT_004487.19 NG_015993.1
Ensembl:   ENSG00000116785
Entrez:   10878
UniGene:   Hs.709217
Chromosome Mapping:   1q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.650000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download