PrimePCR™ Probe Assay: MORN1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: MORN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MORN1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050930
Assay Design:   exonic
Chromosome Location:   1:2317266-2318876question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000334504 ENST00000372216 ENST00000394872 ENST00000545689 ENST00000538570 ENST00000393158 ENST00000302251 ENST00000378531 ENST00000378529 ENST00000449373 ENST00000378525 ENST00000555427 ENST00000555147 ENST00000556922 ENST00000310961 ENST00000035383 ENST00000292303 ENST00000445772 ENST00000343801 ENST00000370768 ENST00000370767 ENST00000436586 ENST00000222792 ENST00000435288 ENST00000375846 ENST00000304141

Gene Information

Description Not Available

Gene Symbol:   MORN1
Gene Name:   MORN repeat containing 1
Aliases:   FLJ13941
RefSeq:   NG_016128.1 NC_000001.10 NT_004350.19
Ensembl:   ENSG00000116151
Entrez:   79906
Chromosome Mapping:   1p36.33-p36.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.940000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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