PrimePCR™ Probe Assay: DCAF17, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050905
Assay Design:   exonic
Chromosome Location:   2:172337490-172337629question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000375255 ENST00000339506 ENST00000431110 ENST00000539783

Gene Information

This gene encodes a nuclear transmembrane protein that associates with cullin 4A/damaged DNA binding protein 1 ubiquitin ligase complex. Mutations in this gene are associated with Woodhouse-Sakati syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq Sep 2009]

Gene Symbol:   DCAF17
Gene Name:   DDB1 and CUL4 associated factor 17
Aliases:   C2orf37, DKFZp781C2086, FLJ13096, FLJ44210
RefSeq:   NC_000002.11 NT_005403.17 NG_013038.1
Ensembl:   ENSG00000115827
Entrez:   80067
UniGene:   Hs.659439
Chromosome Mapping:   2q31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.160000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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