PrimePCR™ Probe Assay: SLC5A7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050896
Assay Design:   exonic
Chromosome Location:   2:108627186-108627283question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000540517 ENST00000264047 ENST00000409059

Gene Information

Choline is a direct precursor of acetylcholine (ACh) a neurotransmitter of the central and peripheral nervous system that regulates a variety of autonomic cognitive and motor functions. SLC5A7 is a Na(+)- and Cl(-)- dependent high-affinity transporter that mediates the uptake of choline for acetylcholine synthesis in cholinergic neurons (Apparsundaram et al. 2000 [PubMed 11027560]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC5A7
Gene Name:   solute carrier family 5 (choline transporter), member 7
Aliases:   CHT, CHT1, MGC126299, MGC126300, hCHT
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000115665
Entrez:   60482
UniGene:   Hs.287758
Chromosome Mapping:   2q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.790000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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