PrimePCR™ Probe Assay: COQ10B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050882
Assay Design:   exonic
Chromosome Location:   2:198338703-198338819question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000263960 ENST00000409398 ENST00000409010 ENST00000545340

Gene Information

Description Not Available

Gene Symbol:   COQ10B
Gene Name:   coenzyme Q10 homolog B (S. cerevisiae)
Aliases:   FLJ13448
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000115520
Entrez:   80219
UniGene:   Hs.606592
Chromosome Mapping:   2q33.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.840000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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