PrimePCR™ Probe Assay: LOXL3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050863
Assay Design:   exonic
Chromosome Location:   2:74777369-74779467question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000413469 ENST00000409986 ENST00000409549 ENST00000393937 ENST00000409249 ENST00000264094

Gene Information

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation senescence tumor suppression cell growth control and chemotaxis to each member of the family. Alternatively spliced transcript variants of this gene have been reported but their full-length nature has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   LOXL3
Gene Name:   lysyl oxidase-like 3
Aliases:   LOXL
RefSeq:   NC_000002.11 NG_012163.1 NT_022184.15
Ensembl:   ENSG00000115318
Entrez:   84695
UniGene:   Hs.469045
Chromosome Mapping:   2p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.840000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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