PrimePCR™ Probe Assay: RTN4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050861
Assay Design:   exonic
Chromosome Location:   2:55209689-55214631question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000394609 ENST00000405240 ENST00000357376 ENST00000337526 ENST00000317610 ENST00000357732 ENST00000394611 ENST00000404909 ENST00000438462 ENST00000402434 ENST00000354474

Gene Information

This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   RTN4
Gene Name:   reticulon 4
Aliases:   ASY, NI220/250, NOGO, NOGO-A, NOGOC, NSP, NSP-CL, Nbla00271, Nbla10545, Nogo-B, Nogo-C, RTN-X, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000115310
Entrez:   57142
UniGene:   Hs.731531
Chromosome Mapping:   2p16.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.710000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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