PrimePCR™ Probe Assay: STEAP3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050837
Assay Design:   exonic
Chromosome Location:   2:120005409-120005520question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000393110 ENST00000393106 ENST00000409811 ENST00000393107 ENST00000393108 ENST00000354888 ENST00000425223 ENST00000450943

Gene Information

Description Not Available

Gene Symbol:   STEAP3
Gene Name:   STEAP family member 3
Aliases:   STMP3, TSAP6, dudlin-2
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000115107
Entrez:   55240
UniGene:   Hs.647822
Chromosome Mapping:   2q14.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997000
y-intercept 36.300000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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