PrimePCR™ Probe Assay: NEK4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050830
Assay Design:   exonic
Chromosome Location:   3:52786096-52786241question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000233027 ENST00000383721 ENST00000461689 ENST00000535191

Gene Information

Description Not Available

Gene Symbol:   NEK4
Gene Name:   NIMA (never in mitosis gene a)-related kinase 4
Aliases:   MGC33171, NRK2, STK2, pp12301
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000114904
Entrez:   6787
UniGene:   Hs.631921
Chromosome Mapping:   3p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.890000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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