PrimePCR™ Probe Assay: SLC25A36, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050796
Assay Design:   exonic
Chromosome Location:   3:140675420-140675528question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000446041 ENST00000507429 ENST00000324194 ENST00000453248 ENST00000513887

Gene Information

Description Not Available

Gene Symbol:   SLC25A36
Gene Name:   solute carrier family 25, member 36
Aliases:   DKFZp564C053, FLJ10618
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000114120
Entrez:   55186
UniGene:   Hs.144130
Chromosome Mapping:   3q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 35.850000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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