PrimePCR™ Probe Assay: HRG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050788
Assay Design:   exonic
Chromosome Location:   3:186395758-186395907question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000232003

Gene Information

This histidine-rich glycoprotein contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme dyes and divalent metal ions. It can inhibit rosette formation and interacts with heparin thrombospondin and plasminogen. Two of the protein's effects the inhibition of fibrinolysis and the reduction of inhibition of coagulation indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. [provided by RefSeq Jul 2008]

Gene Symbol:   HRG
Gene Name:   histidine-rich glycoprotein
Aliases:   DKFZp779H1622, HPRG, HRGP
RefSeq:   NC_000003.11 NG_021485.1 NT_005612.16
Ensembl:   ENSG00000113905
Entrez:   3273
UniGene:   Hs.1498
Chromosome Mapping:   3q27

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 35.320000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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