PrimePCR™ Probe Assay: IRX4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050758
Assay Design:   exonic
Chromosome Location:   5:1880847-1881931question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000231357 ENST00000505790 ENST00000513692 ENST00000511126

Gene Information

Description Not Available

Gene Symbol:   IRX4
Gene Name:   iroquois homeobox 4
Aliases:   IRXA3, MGC131996
RefSeq:   NC_000005.9 NT_006576.16
Ensembl:   ENSG00000113430
Entrez:   50805
UniGene:   Hs.196927
Chromosome Mapping:   5p15.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.510000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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