PrimePCR™ Probe Assay: RASGRF2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050751
Assay Design:   exonic
Chromosome Location:   5:80522030-80522169question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000265080

Gene Information

RAS GTPases cycle between an inactive GDP-bound state and an active GTP-bound state. This gene encodes a calcium-regulated nucleotide exchange factor activating both RAS and RAS-related protein RAC1 through the exchange of bound GDP for GTP thereby coordinating the signaling of distinct mitogen-activated protein kinase pathways. [provided by RefSeq Oct 2011]

Gene Symbol:   RASGRF2
Gene Name:   Ras protein-specific guanine nucleotide-releasing factor 2
Aliases:   DKFZp781H1715, GRF2, RAS-GRF2
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000113319
Entrez:   5924
UniGene:   Hs.162129
Chromosome Mapping:   5q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.710000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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