PrimePCR™ Probe Assay: HAND1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050736
Assay Design:   exonic
Chromosome Location:   5:153854818-153854961question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000231121

Gene Information

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members the HAND proteins which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion they function in the formation of the right ventricle and aortic arch arteries implicating them as mediators of congenital heart disease. In addition it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq Jul 2008]

Gene Symbol:   HAND1
Gene Name:   heart and neural crest derivatives expressed 1
Aliases:   Hxt, Thing1, bHLHa27, eHand
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000113196
Entrez:   9421
UniGene:   Hs.152531
Chromosome Mapping:   5q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.130000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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