PrimePCR™ Probe Assay: FAF2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050735
Assay Design:   exonic
Chromosome Location:   5:175935343-175935463question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000261942

Gene Information

The protein encoded by this gene is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals. It may play a role in regulating the resistance to apoptosis that is observed in T cells and eosinophils of AD patients. [provided by RefSeq Jul 2008]

Gene Symbol:   FAF2
Gene Name:   Fas associated factor family member 2
Aliases:   ETEA, KIAA0887, UBXD8, UBXN3B
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000113194
Entrez:   23197
UniGene:   Hs.484242
Chromosome Mapping:   5q35.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.250000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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