PrimePCR™ Probe Assay: GABRR2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050675
Assay Design:   exonic
Chromosome Location:   6:89967262-89967386question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000402938 ENST00000602399

Gene Information

GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA receptors which are ligand-gated chloride channels. The protein encoded by this gene is a member of the rho subunit family and is a component of the GABA receptor complex. [provided by RefSeq Jul 2008]

Gene Symbol:   GABRR2
Gene Name:   gamma-aminobutyric acid (GABA) receptor, rho 2
Aliases:   Not Available
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000111886
Entrez:   2570
UniGene:   Hs.719891
Chromosome Mapping:   6q14-q21,6q13-q16.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.580000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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