PrimePCR™ Probe Assay: MAN1A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050674
Assay Design:   exonic
Chromosome Location:   6:119569506-119623161question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000368468 ENST00000368466

Gene Information

This gene encodes a class I mammalian Golgi 12-mannosidase which is a type II transmembrane protein. This protein catalyzes the removal of 3 distinct mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides and belongs to family 47 of glycosyl hydrolases. [provided by RefSeq Jul 2008]

Gene Symbol:   MAN1A1
Gene Name:   mannosidase, alpha, class 1A, member 1
Aliases:   HUMM3, HUMM9, MAN9
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000111885
Entrez:   4121
UniGene:   Hs.102788
Chromosome Mapping:   6q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.550000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download