PrimePCR™ Probe Assay: GCNT2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050672
Assay Design:   exonic
Chromosome Location:   6:10626895-10627028question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000410107 ENST00000495262 ENST00000379597 ENST00000316170 ENST00000265012

Gene Information

This gene encodes the enzyme responsible for formation of the blood group I antigen. The i and I antigens are distinguished by linear and branched poly-N-acetyllactosaminoglycans respectively. The encoded protein is the I-branching enzyme a beta-16-N-acetylglucosaminyltransferase responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   GCNT2
Gene Name:   glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group)
Aliases:   CCAT, GCNT2C, GCNT5, IGNT, II, MGC163396, NACGT1, NAGCT1, ULG3, bA360O19.2, bA421M1.1
RefSeq:   NC_000006.11 NG_007469.2 NT_007592.15
Ensembl:   ENSG00000111846
Entrez:   2651
UniGene:   Hs.519884
Chromosome Mapping:   6p24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.640000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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