PrimePCR™ Probe Assay: SLCO1B3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050654
Assay Design:   exonic
Chromosome Location:   12:21015779-21028267question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000256958 ENST00000540853 ENST00000381545 ENST00000544370 ENST00000553473 ENST00000261196 ENST00000381541 ENST00000540229 ENST00000421593 ENST00000554957

Gene Information

This gene encodes a liver-specific member of the organic anion transporter family. The encoded protein is a transmembrane receptor that mediates the sodium-independent uptake of endogenous and xenobiotic compounds and plays a critical role in bile acid and bilirubin transport. Mutations in this gene are a cause of Rotor type hyperbilirubinemia. [provided by RefSeq Feb 2012]

Gene Symbol:   SLCO1B3
Gene Name:   solute carrier organic anion transporter family, member 1B3
Aliases:   LST-3TM13, LST3, OATP1B3, OATP8, SLC21A8
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000111700
Entrez:   28234
UniGene:   Hs.504966
Chromosome Mapping:   12p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.920000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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