PrimePCR™ Probe Assay: IFNG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050640
Assay Design:   exonic
Chromosome Location:   12:68548835-68548962question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000229135

Gene Information

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq Nov 2009]

Gene Symbol:   IFNG
Gene Name:   interferon, gamma
Aliases:   IFG, IFI
RefSeq:   NC_000012.11 NG_015840.1 NT_029419.12
Ensembl:   ENSG00000111537
Entrez:   3458
UniGene:   Hs.856
Chromosome Mapping:   12q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.770000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download