PrimePCR™ Probe Assay: PRR4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050616
Assay Design:   exonic
Chromosome Location:   12:10998538-10999745question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000576804 ENST00000570336 ENST00000573190 ENST00000544994 ENST00000228811 ENST00000540107 ENST00000258886 ENST00000358625 ENST00000425041 ENST00000482352 ENST00000312960 ENST00000251472 ENST00000576999 ENST00000574424 ENST00000574037

Gene Information

This gene encodes a member of the proline-rich protein family that lacks a conserved repetitive domain. This protein may play a role in protective functions in the eye. Alternative splicing result in multiple transcript variants. Read-through transcription also exists between this gene and the upstream PRH1 (proline-rich protein HaeIII subfamily 1) gene. [provided by RefSeq Feb 2011]

Gene Symbol:   PRR4
Gene Name:   proline rich 4 (lacrimal)
Aliases:   DKFZp779L1763, FLJ18757, LPRP, PROL4
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000111215
Entrez:   11272
UniGene:   Hs.732031
Chromosome Mapping:   12p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.360000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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