PrimePCR™ Probe Assay: ELK3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050611
Assay Design:   exonic
Chromosome Location:   12:96617368-96617466question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000228741 ENST00000547249 ENST00000552142 ENST00000547860

Gene Information

The protein encoded by this gene is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras but activates transcription when Ras is present. [provided by RefSeq Jul 2008]

Gene Symbol:   ELK3
Gene Name:   ELK3, ETS-domain protein (SRF accessory protein 2)
Aliases:   ERP, NET, SAP2
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000111145
Entrez:   2004
UniGene:   Hs.46523
Chromosome Mapping:   12q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.890000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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