PrimePCR™ Probe Assay: GTF2H1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050590
Assay Design:   exonic
Chromosome Location:   11:18387332-18387452question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000368491 ENST00000434604 ENST00000360290 ENST00000392500 ENST00000419517 ENST00000368488 ENST00000453096 ENST00000534641 ENST00000265963 ENST00000530496 ENST00000526630

Gene Information

Description Not Available

Gene Symbol:   GTF2H1
Gene Name:   general transcription factor IIH, polypeptide 1, 62kDa
Aliases:   BTF2, TFB1, TFIIH
RefSeq:   NC_000011.9 NG_008877.1 NT_009237.18
Ensembl:   ENSG00000110768
Entrez:   2965
UniGene:   Hs.577202
Chromosome Mapping:   11p15.1-p14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.740000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download