PrimePCR™ Probe Assay: PITPNM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PITPNM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PITPNM1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050585
Assay Design:   exonic
Chromosome Location:   11:67269553-67269865question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000244289 ENST00000324096 ENST00000544059 ENST00000401533 ENST00000407684 ENST00000449407 ENST00000448662 ENST00000534749 ENST00000436757 ENST00000533391 ENST00000532703 ENST00000528559 ENST00000356404

Gene Information

PITPNM1 belongs to a family of membrane-associated phosphatidylinositol transfer domain-containing proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein (Ocaka et al. 2005 [PubMed 15627748]).[supplied by OMIM Mar 2008]

Gene Symbol:   PITPNM1
Gene Name:   phosphatidylinositol transfer protein, membrane-associated 1
Aliases:   DRES9, FLJ44997, NIR2, PITPNM, RDGB, RDGB1, RDGBA, RDGBA1, Rd9
RefSeq:   NC_000011.9 NG_008969.1 NT_167190.1
Ensembl:   ENSG00000110697
Entrez:   9600
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.240000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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