PrimePCR™ Probe Assay: CRTAM, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050560
Assay Design:   exonic
Chromosome Location:   11:122742076-122742219question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000227348 ENST00000533709

Gene Information

The CRTAM gene is upregulated in CD4 (see MIM 186940)-positive and CD8 (see CD8A; MIM 186910)-positive T cells and encodes a type I transmembrane protein with V and C1-like Ig domains (Yeh et al. 2008 [PubMed 18329370]).[supplied by OMIM Feb 2009]

Gene Symbol:   CRTAM
Gene Name:   cytotoxic and regulatory T cell molecule
Aliases:   Not Available
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000109943
Entrez:   56253
UniGene:   Hs.159523
Chromosome Mapping:   11q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.790000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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