PrimePCR™ Probe Assay: CLNK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050548
Assay Design:   exonic
Chromosome Location:   4:10542129-10543657question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000226951 ENST00000507719 ENST00000442825

Gene Information

MIST is a member of the SLP76 family of adaptors (see LCP2 MIM 601603; BLNK MIM 604515). MIST plays a role in the regulation of immunoreceptor signaling including PLC-gamma (PLCG1; MIM 172420)-mediated B cell antigen receptor (BCR) signaling and FC-epsilon R1 (see FCER1A MIM 147140)-mediated mast cell degranulation (Cao et al. 1999 [PubMed 10562326]; Goitsuka et al. 2000 2001 [PubMed 10744659] [PubMed 11463797]).[supplied by OMIM Mar 2008]

Gene Symbol:   CLNK
Gene Name:   cytokine-dependent hematopoietic cell linker
Aliases:   MGC35197, MIST
RefSeq:   NC_000004.11 NT_006316.16
Ensembl:   ENSG00000109684
Entrez:   116449
UniGene:   Hs.678910
Chromosome Mapping:   4p16.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.300000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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