PrimePCR™ Probe Assay: NEIL3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050547
Assay Design:   exonic
Chromosome Location:   4:178283715-178283863question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000264596

Gene Information

NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al. 2002 [PubMed 12509226]).[supplied by OMIM Mar 2008]

Gene Symbol:   NEIL3
Gene Name:   nei endonuclease VIII-like 3 (E. coli)
Aliases:   FGP2, FLJ10858, FPG2, NEI3, hFPG2, hNEI3
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000109674
Entrez:   55247
UniGene:   Hs.405467
Chromosome Mapping:   4q34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.450000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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