PrimePCR™ Probe Assay: KRT37, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050487
Assay Design:   exonic
Chromosome Location:   17:39577624-39577773question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000225550 ENST00000574588 ENST00000246646

Gene Information

The protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. [provided by RefSeq Jul 2008]

Gene Symbol:   KRT37
Gene Name:   keratin 37
Aliases:   HA7, KRTHA7, hHa7
RefSeq:   NC_000017.10 NG_000018.2 NG_012294.1 NT_010783.15 NW_003315953.1
Ensembl:   ENSG00000108417
Entrez:   8688
UniGene:   Hs.673852
Chromosome Mapping:   17q12-q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.780000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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