PrimePCR™ Probe Assay: PBLD, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050472
Assay Design:   exonic
Chromosome Location:   10:70048249-70048407question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000409549 ENST00000409249 ENST00000264094 ENST00000336578 ENST00000309049 ENST00000277795 ENST00000358769 ENST00000432941

Gene Information

Description Not Available

Gene Symbol:   PBLD
Gene Name:   phenazine biosynthesis-like protein domain containing
Aliases:   FLJ14767, FLJ35507, MAWBP, MAWDBP
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000108187
Entrez:   64081
UniGene:   Hs.198158
Chromosome Mapping:   10pter-q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.550000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download