PrimePCR™ Probe Assay: PPP3CB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050457
Assay Design:   exonic
Chromosome Location:   10:75230956-75231326question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000360663 ENST00000342558 ENST00000394829 ENST00000394828 ENST00000394822 ENST00000545874

Gene Information

Description Not Available

Gene Symbol:   PPP3CB
Gene Name:   protein phosphatase 3, catalytic subunit, beta isozyme
Aliases:   CALNA2, CALNB, CNA2, PP2Bbeta
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000107758
Entrez:   5532
UniGene:   Hs.500067
Chromosome Mapping:   10q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.530000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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