PrimePCR™ Probe Assay: DNMBP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050453
Assay Design:   exonic
Chromosome Location:   10:101639790-101639920question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000540316 ENST00000543621 ENST00000342239 ENST00000324109

Gene Information

DNMBP belongs to the DBL (MIM 311030) family of guanine nucleotide exchange factors and plays a role in the regulation of cell junctions (Otani et al. 2006 [PubMed 17015620]).[supplied by OMIM Mar 2008]

Gene Symbol:   DNMBP
Gene Name:   dynamin binding protein
Aliases:   ARHGEF36, KIAA1010, TUBA
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000107554
Entrez:   23268
UniGene:   Hs.500771
Chromosome Mapping:   10q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.600000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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