PrimePCR™ Probe Assay: GLIS3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050444
Assay Design:   exonic
Chromosome Location:   9:4125738-4125845question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000324333 ENST00000381971 ENST00000462164 ENST00000478315 ENST00000478844 ENST00000481827 ENST00000477901

Gene Information

This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells the thyroid eye liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   GLIS3
Gene Name:   GLIS family zinc finger 3
Aliases:   FLJ38999, FLJ90578, MGC33662, ZNF515
RefSeq:   NC_000009.11 NG_011782.1 NT_008413.18
Ensembl:   ENSG00000107249
Entrez:   169792
UniGene:   Hs.162125
Chromosome Mapping:   9p24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.920000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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