PrimePCR™ Probe Assay: MOGAT3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050407
Assay Design:   exonic
Chromosome Location:   7:100841997-100842109question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000223114 ENST00000440203 ENST00000379423

Gene Information

Acyl-CoA:monoacylglycerol acyltransferase (MOGAT; EC 2.3.1.22) catalyzes the synthesis of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA (Cheng et al. 2003 [PubMed 12618427]).[supplied by OMIM Mar 2008]

Gene Symbol:   MOGAT3
Gene Name:   monoacylglycerol O-acyltransferase 3
Aliases:   DC7, DGAT2L7, MGAT3, MGC119203, MGC119204
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000106384
Entrez:   346606
UniGene:   Hs.512217
Chromosome Mapping:   7q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996200
y-intercept 35.960000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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