PrimePCR™ Probe Assay: WASL, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050401
Assay Design:   exonic
Chromosome Location:   7:123322261-123322387question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000223023

Gene Information

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli and interact with multiple proteins. Recent studies have demonstrated that these proteins directly or indirectly associate with the small GTPase Cdc42 known to regulate formation of actin filaments and the cytoskeletal organizing complex Arp2/3. The WASL gene product is a homolog of WAS protein however unlike the latter it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly and induce formation of long actin microspikes. [provided by RefSeq Jul 2008]

Gene Symbol:   WASL
Gene Name:   Wiskott-Aldrich syndrome-like
Aliases:   DKFZp779G0847, MGC48327, N-WASP, NWASP
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000106299
Entrez:   8976
UniGene:   Hs.731519
Chromosome Mapping:   7q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.410000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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