PrimePCR™ Probe Assay: PTPRZ1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050399
Assay Design:   exonic
Chromosome Location:   7:121650983-121651117question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000449182 ENST00000393386

Gene Information

This gene encodes a member of the receptor protein tyrosine phosphatase family. Expression of this gene is restricted to the central nervous system (CNS) and it may be involved in the regulation of specific developmental processes in the CNS. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq May 2011]

Gene Symbol:   PTPRZ1
Gene Name:   protein tyrosine phosphatase, receptor-type, Z polypeptide 1
Aliases:   HPTPZ, HPTPzeta, PTP-ZETA, PTP18, PTPRZ, PTPZ, RPTPB, RPTPbeta, phosphacan
RefSeq:   NC_000007.13 NG_023266.1 NT_007933.15
Ensembl:   ENSG00000106278
Entrez:   5803
UniGene:   Hs.489824
Chromosome Mapping:   7q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.090000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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