PrimePCR™ Probe Assay: TTC26, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050376
Assay Design:   exonic
Chromosome Location:   7:138874285-138874397question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000495038 ENST00000478836 ENST00000464848 ENST00000343187 ENST00000430935

Gene Information

Description Not Available

Gene Symbol:   TTC26
Gene Name:   tetratricopeptide repeat domain 26
Aliases:   DYF13, FLJ12571, MGC163211, dyf-13
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000105948
Entrez:   79989
UniGene:   Hs.644553
Chromosome Mapping:   7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.660000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download