PrimePCR™ Probe Assay: ZC3HAV1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ZC3HAV1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZC3HAV1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050375
Assay Design:   exonic
Chromosome Location:   7:138758666-138758755question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000242351 ENST00000464606 ENST00000460845 ENST00000471652

Gene Information

This gene encodes a CCCH-type zinc finger protein that is thought to prevent infection by retroviruses. Studies of the rat homolog indicate that the protein may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants each encoding distinct isoforms are described. [provided by RefSeq Jul 2008]

Gene Symbol:   ZC3HAV1
Gene Name:   zinc finger CCCH-type, antiviral 1
Aliases:   DKFZp686F2052, DKFZp686H1869, DKFZp686O19171, FLB6421, FLJ13288, MGC48898, PARP13, ZAP, ZC3H2, ZC3HDC2
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000105939
Entrez:   56829
Chromosome Mapping:   7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.470000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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