This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: ERF, Human
PrimePCR™ Template for Probe Assay: ERF, Human
Members of the ETS family of transcription factors such as ERF regulate cell proliferation and differentiation. They share a highly conserved DNA-binding domain the ETS domain that recognizes the sequence GGAA/T (de Castro et al. 1997 [PubMed 9192842]). For further information on ETS transcription factors see ETS1 (MIM 164720).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.