PrimePCR™ Probe Assay: KDELR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KDELR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KDELR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050332
Assay Design:   exonic
Chromosome Location:   19:48892858-48892964question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000330720 ENST00000597017 ENST00000600980

Gene Information

Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal usually lys-asp-glu-leu (KDEL) in animal cells and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes and binds the tetrapeptide-containing protein and returns it to the ER. In yeast the sorting receptor encoded by a single gene ERD2 which is a seven-transmembrane protein. Unlike yeast several human homologs of the ERD2 gene constituting the KDEL receptor gene family have been described. The protein encoded by this gene was the first member of the family to be identified and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. [provided by RefSeq Jul 2008]

Gene Symbol:   KDELR1
Gene Name:   KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1
Aliases:   ERD2, ERD2.1, HDEL, PM23
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000105438
Entrez:   10945
Chromosome Mapping:   19q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.730000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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